Cyagen ITS (Insulin, Transferrin, Selenium) Cell Culture Supplement (100×) is a liquid reagent to be added into serum-reduced cell culture medium to stimulate proliferation of a variety of cells.
- Improved ability to support cell proliferation in low serum media condition.
- Batch-to-batch consistency.
The ingredients are resolved in pH 7.0~7.2 EBSS solution.
Ingredients : Weight/ 5mL
Human Recombinant insulin : 3.125 mg
Human Transferrin : 3.125 mg
Selenous Acid : 3.125 μg
BSA : 0.625 g
Linoleic acid : 2.675 mg
- Store at 2-8oC in dark for up to one year.
- Avoid repeated warm-cooling and freeze-thawing for optimal performance.
Standard evaluation includes:
1. Sterility test (bacteria, fungi and mycoplasma)
2. pH test
1 mL ITS Cell Culture Supplement (100×) is added to 99 mL low serum medium for cell culture.