Cyagen ITS (Insulin, Transferrin, Selenium) Cell Culture Supplement (100×) is a liquid reagent to be added into serum-reduced cell culture medium to stimulate proliferation of a variety of cells.
- Improved ability to support cell proliferation in low-serum media condition.
- Batch-to-batch consistency.
The ingredients are resolved in pH 7.0~7.2 EBSS solution.
Ingredients : Weight/ 10mL
Human Recombinant insulin : 6.25 mg
Human Transferrin : 6.25 mg
Selenous Acid : 6.25 μg
BSA : 1.25 g
Linoleic acid : 5.35 mg
- Store at 2-8oC in the dark for up to one year.
- Avoid repeated warm-cooling and freeze-thawing for optimal performance.
Standard evaluation includes:
1. Sterility test (bacteria, fungi and mycoplasma)
2. pH test
1 mL ITS Cell Culture Supplement (100×) is added to 99 mL low-serum medium for cell culture.